[HTML][HTML] Inhibition of infection of incoming HIV-1 virus by RNA-cleaving DNA enzyme
X Zhang, Y Xu, H Ling, T Hattori - FEBS letters, 1999 - Elsevier
X Zhang, Y Xu, H Ling, T Hattori
FEBS letters, 1999•ElsevierNine different DNA enzymes (DzV3-n, n= 1–9) targeting the V3 loop region of HIV-1 HXB2
were synthesized. One of those, DzV3-9, efficiently cleaved the target in the conserved
sequence in the RNA transcript in vitro. DzV3-9 was stable in the cells and inhibited
replication of both NL432 and SF162 strains in U87 cells expressing CD4 and co-receptors.
The inhibitory effect of DNAzyme on incoming HIV-1 was also demonstrated with
pseudotype virions generated by NL432-based luciferase reporter genes. Thus, an efficient …
were synthesized. One of those, DzV3-9, efficiently cleaved the target in the conserved
sequence in the RNA transcript in vitro. DzV3-9 was stable in the cells and inhibited
replication of both NL432 and SF162 strains in U87 cells expressing CD4 and co-receptors.
The inhibitory effect of DNAzyme on incoming HIV-1 was also demonstrated with
pseudotype virions generated by NL432-based luciferase reporter genes. Thus, an efficient …
Nine different DNA enzymes (DzV3-n, n=1–9) targeting the V3 loop region of HIV-1 HXB2 were synthesized. One of those, DzV3-9, efficiently cleaved the target in the conserved sequence in the RNA transcript in vitro. DzV3-9 was stable in the cells and inhibited replication of both NL432 and SF162 strains in U87 cells expressing CD4 and co-receptors. The inhibitory effect of DNAzyme on incoming HIV-1 was also demonstrated with pseudotype virions generated by NL432-based luciferase reporter genes. Thus, an efficient, stable DNAzyme against a functionally important region of HIV-1 was identified, and it may be useful for prevention of HIV-1 infection.
Elsevier
