Calcitonin directly acts on mouse osteoblastic MC3T3-E1 cells to stimulate mRNA expression of c-fos, insulin-like growth factor-1 and osteoblastic phenotypes (type 1 …
T Kobayashi, T Sugimoto, K Saijoh, M Fukase… - Biochemical and …, 1994 - Elsevier
T Kobayashi, T Sugimoto, K Saijoh, M Fukase, K Chihara
Biochemical and biophysical research communications, 1994•ElsevierThe present study was performed to examine whether calcitonin directly acted on mouse
osteoblastic MC3T3-E1 cells to stimulate the mRNA expression of insulin-like growth factor-
1 (IGF-1) and c-fos, followed by an increase in their proliferation and differentiation. Eel
calcitonin increased [3 H] thymidine incorporation and alkaline phosphatase activity as well
as the mRNA expression of type 1 collagen and osteocalcin which were characteristic of
osteoblasts. Eel calcitonin (10− 8 M) induced c-fos mRNA transiently after its addition …
osteoblastic MC3T3-E1 cells to stimulate the mRNA expression of insulin-like growth factor-
1 (IGF-1) and c-fos, followed by an increase in their proliferation and differentiation. Eel
calcitonin increased [3 H] thymidine incorporation and alkaline phosphatase activity as well
as the mRNA expression of type 1 collagen and osteocalcin which were characteristic of
osteoblasts. Eel calcitonin (10− 8 M) induced c-fos mRNA transiently after its addition …
Abstract
The present study was performed to examine whether calcitonin directly acted on mouse osteoblastic MC3T3-E1 cells to stimulate the mRNA expression of insulin-like growth factor-1 (IGF-1) and c-fos, followed by an increase in their proliferation and differentiation. Eel calcitonin increased [3H]thymidine incorporation and alkaline phosphatase activity as well as the mRNA expression of type 1 collagen and osteocalcin which were characteristic of osteoblasts. Eel calcitonin (10−8M) induced c-fos mRNA transiently after its addition, followed by gene expression of IGF-1, an important autocrine/paracrine growth factor in the regulation of osteoblastic proliferation. We first demonstrated that calcitonin directly acted on osteoblasts to stimulate transcription of c-fos and IGF-1 genes as well as functional phenotypes including type 1 collagen and osteocalcin.
Elsevier
