[HTML][HTML] Functional re-expression of laminin-5 in laminin-γ2-deficient human keratinocytes modifies cell morphology, motility, and adhesion
L Gagnoux-Palacios, J Vailly… - Journal of Biological …, 1996 - ASBMB
Herlitz junctional epidermolysis bullosa (H-JEB) is characterized by a reduced adherence of
keratinocytes consequent to deficient expression of the extracellular adhesive ligand laminin-
5. To complement the genetic defect causing H-JEB, we transferred an eukaryotic cassette
expressing the cDNA for the γ2 chain of laminin-5 into H-JEB keratinocytes in which the
expression of the polypeptide is hampered by a homozygous mutation generating a
premature termination codon. Transfection using adenovirus-polylysin-transferrin-DNA …
keratinocytes consequent to deficient expression of the extracellular adhesive ligand laminin-
5. To complement the genetic defect causing H-JEB, we transferred an eukaryotic cassette
expressing the cDNA for the γ2 chain of laminin-5 into H-JEB keratinocytes in which the
expression of the polypeptide is hampered by a homozygous mutation generating a
premature termination codon. Transfection using adenovirus-polylysin-transferrin-DNA …
