[HTML][HTML] Identification of common germinal-center B-cell precursors in two patients with both Hodgkin's disease and non-Hodgkin's lymphoma
A Bräuninger, ML Hansmann, JG Strickler… - … England Journal of …, 1999 - Mass Medical Soc
New England Journal of Medicine, 1999•Mass Medical Soc
Background Hodgkin's disease and non-Hodgkin's B-cell lymphoma occasionally occur in
the same patient. The identification of a common precursor of the two types of lymphoma
would show definitively that Reed–Sternberg cells originate from B cells. Methods We
studied lymphomas from two patients, one with a composite lymphoma (classic Hodgkin's
disease and a follicular lymphoma in the same lymph node) and the other with a T-cell–rich
B-cell lymphoma that was followed by classic Hodgkin's disease. Single Reed–Sternberg …
the same patient. The identification of a common precursor of the two types of lymphoma
would show definitively that Reed–Sternberg cells originate from B cells. Methods We
studied lymphomas from two patients, one with a composite lymphoma (classic Hodgkin's
disease and a follicular lymphoma in the same lymph node) and the other with a T-cell–rich
B-cell lymphoma that was followed by classic Hodgkin's disease. Single Reed–Sternberg …
Background
Hodgkin's disease and non-Hodgkin's B-cell lymphoma occasionally occur in the same patient. The identification of a common precursor of the two types of lymphoma would show definitively that Reed–Sternberg cells originate from B cells.
Methods
We studied lymphomas from two patients, one with a composite lymphoma (classic Hodgkin's disease and a follicular lymphoma in the same lymph node) and the other with a T-cell–rich B-cell lymphoma that was followed by classic Hodgkin's disease. Single Reed–Sternberg cells and non-Hodgkin's lymphoma cells from frozen sections were micromanipulated. The rearranged immunoglobulin variable-region genes (V genes) of the heavy and light chains were amplified by the polymerase chain reaction from genomic DNA and sequenced.
Results
In both patients, the Reed–Sternberg cells were related clonally to the non-Hodgkin's lymphoma B cells. The V genes carried somatic mutations (a hallmark of germinal-center B cells and their descendants). In both patients, some somatic mutations were shared by the Reed–Sternberg and non-Hodgkin's lymphoma cells, whereas other somatic mutations were found exclusively in one or the other cell type.
Conclusions
In two patients with classic Hodgkin's disease and non-Hodgkin's B-cell lymphoma, we identified a common B-cell precursor, probably a germinal-center B cell, for both lymphomas. This finding suggests that the two types of lymphoma underwent both shared and distinct transforming events and provides proof of the B-cell derivation of Reed–Sternberg cells in classic Hodgkin's disease.
The New England Journal Of Medicine