Mutational analysis of the fractalkine chemokine domain: basic amino acid residues differentially contribute to CX3CR1 binding, signaling, and cell adhesion
JK Harrison, AM Fong, PAW Swain, S Chen… - Journal of Biological …, 2001 - ASBMB
Fractalkine (FKN/CX3CL1) is a unique member of the chemokine gene family and contains
a chemokine domain (CD), a mucin-like stalk, a single transmembrane region, and a short
intracellular C terminus. This structural distinction affords FKN the property of mediating
capture and firm adhesion of FKN receptor (CX3CR1)-expressing cells under physiological
flow conditions. Shed forms of FKN also exist, and these promote chemotaxis of CX3CR1-
expressing leukocytes. The goal of the present study was to identify specific residues within …
a chemokine domain (CD), a mucin-like stalk, a single transmembrane region, and a short
intracellular C terminus. This structural distinction affords FKN the property of mediating
capture and firm adhesion of FKN receptor (CX3CR1)-expressing cells under physiological
flow conditions. Shed forms of FKN also exist, and these promote chemotaxis of CX3CR1-
expressing leukocytes. The goal of the present study was to identify specific residues within …
