[HTML][HTML] Characterization of the Acyl-CoA synthetase activity of purified murine fatty acid transport protein 1
AM Hall, AJ Smith, DA Bernlohr - Journal of Biological Chemistry, 2003 - ASBMB
Fatty acid transport protein 1 (FATP1) is an∼ 63-kDa plasma membrane protein that
facilitates the influx of fatty acids into adipocytes as well as skeletal and cardiac myocytes.
Previous studies with FATP1 expressed in COS1 cell extracts suggested that FATP1 exhibits
very long chain acyl-CoA synthetase (ACS) activity and that such activity may be linked to
fatty acid transport. To address the enzymatic activity of the isolated protein, murine FATP1
and ACS1 were engineered to contain a C-terminal Myc-His tag expressed in COS1 cells …
facilitates the influx of fatty acids into adipocytes as well as skeletal and cardiac myocytes.
Previous studies with FATP1 expressed in COS1 cell extracts suggested that FATP1 exhibits
very long chain acyl-CoA synthetase (ACS) activity and that such activity may be linked to
fatty acid transport. To address the enzymatic activity of the isolated protein, murine FATP1
and ACS1 were engineered to contain a C-terminal Myc-His tag expressed in COS1 cells …