Deficiencies in Pro-thyrotropin-releasing Hormone Processing and Abnormalities in Thermoregulation in Cpefat/fatMice
EA Nillni, W Xie, L Mulcahy, VC Sanchez… - Journal of Biological …, 2002 - ASBMB
Cpe fat/fat mice are obese, diabetic, and infertile. They have a mutation in carboxypeptidase
E (CPE), an enzyme that converts prohormone intermediates to bioactive peptides. The Cpe
fat mutation leads to rapid degradation of the enzyme. To test whether pro-thyrotropin-
releasing hormone (TRH) conversion to TRH involves CPE, processing was examined in
theCpe fat/fat mouse. Hypothalamic TRH is depressed by at least 75% compared with wild-
type controls. Concentrations of pro-TRH forms are increased in homozygotes. TRH-[Gly 4 …
E (CPE), an enzyme that converts prohormone intermediates to bioactive peptides. The Cpe
fat mutation leads to rapid degradation of the enzyme. To test whether pro-thyrotropin-
releasing hormone (TRH) conversion to TRH involves CPE, processing was examined in
theCpe fat/fat mouse. Hypothalamic TRH is depressed by at least 75% compared with wild-
type controls. Concentrations of pro-TRH forms are increased in homozygotes. TRH-[Gly 4 …
