Macrophage progenitors from mouse bone marrow and spleen differ in their expression of the Ly-6C differentiation antigen.
JM McCormack, PJ Leenen… - Journal of immunology …, 1993 - journals.aai.org
JM McCormack, PJ Leenen, WS Walker
Journal of immunology (Baltimore, Md.: 1950), 1993•journals.aai.orgUsing mAb and magnetic and fluorescence-activated cell sorting, together with culturing in
semisolid culture medium, we show that essentially all splenic macrophage progenitors are
Ly-6ChiMac-1-. By contrast, bone marrow macrophage progenitors were more
heterogeneous in Ly-6C expression, the majority being restricted to the Ly-6CdimMac-1-and
the Ly-6C-Mac-1-subsets. These findings provide immunophenotype evidence that
macrophages in the spleen and bone marrow arise from different progenitors.
semisolid culture medium, we show that essentially all splenic macrophage progenitors are
Ly-6ChiMac-1-. By contrast, bone marrow macrophage progenitors were more
heterogeneous in Ly-6C expression, the majority being restricted to the Ly-6CdimMac-1-and
the Ly-6C-Mac-1-subsets. These findings provide immunophenotype evidence that
macrophages in the spleen and bone marrow arise from different progenitors.
Abstract
Using mAb and magnetic and fluorescence-activated cell sorting, together with culturing in semisolid culture medium, we show that essentially all splenic macrophage progenitors are Ly-6ChiMac-1-. By contrast, bone marrow macrophage progenitors were more heterogeneous in Ly-6C expression, the majority being restricted to the Ly-6CdimMac-1- and the Ly-6C-Mac-1- subsets. These findings provide immunophenotype evidence that macrophages in the spleen and bone marrow arise from different progenitors.
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