Direct evidence of NO production in rat hippocampus and cortex using a new fluorescent indicator: DAF-2 DA
H Kojima, N Nakatsubo, K Kikuchi, Y Urano… - …, 1998 - journals.lww.com
THE biological functions of nitric oxide in the neuronal system remain controversial. Using a
novel fluorescence indicator, DAF-2 DA, for direct detection of NO, we examined both acute
rat brain slices and organotypic culture of brain slices to ascertain NO production sites. The
fluorescence intensity in the CA1 region of the hippocampus was augmented, especially
after stimulation with NMDA, in acute brain slices. This NO production in the CA1 region was
also confirmed in cultured hippocampus. This is the first direct evidence of NO production in …
novel fluorescence indicator, DAF-2 DA, for direct detection of NO, we examined both acute
rat brain slices and organotypic culture of brain slices to ascertain NO production sites. The
fluorescence intensity in the CA1 region of the hippocampus was augmented, especially
after stimulation with NMDA, in acute brain slices. This NO production in the CA1 region was
also confirmed in cultured hippocampus. This is the first direct evidence of NO production in …
