Intrinsic regulation of glucose output by rat liver
WH Glinsmann, EP Hern… - American Journal of …, 1969 - journals.physiology.org
WH Glinsmann, EP Hern, A Lynch
American Journal of Physiology-Legacy Content, 1969•journals.physiology.orgMETHODS Male Sprague-Dawley rats from the National Institutes of Health pathogen-free
colony were fed ad lib. until 2 hr before experimentation, or were fasted 48 hr prior to use.
Their weights ranged from 120 to 150 g. All animals were anesthetized with pentobarbital
sodium (35-40 mg/kg intraperi toneally) lo-15 min prior to perfusion. The method for the
cyclic perfusion of isolated rat livers in situ has been described(17, 18), and, as in previous
experiments, two or four parallel perfusions were run within a single temperature …
colony were fed ad lib. until 2 hr before experimentation, or were fasted 48 hr prior to use.
Their weights ranged from 120 to 150 g. All animals were anesthetized with pentobarbital
sodium (35-40 mg/kg intraperi toneally) lo-15 min prior to perfusion. The method for the
cyclic perfusion of isolated rat livers in situ has been described(17, 18), and, as in previous
experiments, two or four parallel perfusions were run within a single temperature …
METHODS
Male Sprague-Dawley rats from the National Institutes of Health pathogen-free colony were fed ad lib. until 2 hr before experimentation, or were fasted 48 hr prior to use. Their weights ranged from 120 to 150 g. All animals were anesthetized with pentobarbital sodium (35-40 mg/kg intraperi toneally) lo-15 min prior to perfusion.
The method for the cyclic perfusion of isolated rat livers in situ has been described(17, 18), and, as in previous experiments, two or four parallel perfusions were run within a single temperature-controlled(37 C) cabinet. Perfusate flow was maintained between 1.8 and 2.0 ml/g liver per min by a diaphragm pump which minimized hemolysis.
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