A highly sensitive protein-protein interaction assay based on Gaussia luciferase
I Remy, SW Michnick - Nature methods, 2006 - nature.com
I Remy, SW Michnick
Nature methods, 2006•nature.comProtein-fragment complementation assays (PCAs) provide a general strategy to study the
dynamics of protein-protein interactions in vivo and in vitro. The full potential of PCA requires
assays that are fully reversible and sensitive at subendogenous protein expression levels.
We describe a new assay that meets these criteria, based on the Gaussia princeps
luciferase enzyme, demonstrating chemical reversal, and induction and inhibition of a key
interaction linking insulin and TGFβ signaling.
dynamics of protein-protein interactions in vivo and in vitro. The full potential of PCA requires
assays that are fully reversible and sensitive at subendogenous protein expression levels.
We describe a new assay that meets these criteria, based on the Gaussia princeps
luciferase enzyme, demonstrating chemical reversal, and induction and inhibition of a key
interaction linking insulin and TGFβ signaling.
Abstract
Protein-fragment complementation assays (PCAs) provide a general strategy to study the dynamics of protein-protein interactions in vivo and in vitro. The full potential of PCA requires assays that are fully reversible and sensitive at subendogenous protein expression levels. We describe a new assay that meets these criteria, based on the Gaussia princeps luciferase enzyme, demonstrating chemical reversal, and induction and inhibition of a key interaction linking insulin and TGFβ signaling.
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