Insulin-like effect of vanadate on adipocyte glycogen synthase and on phosphorylation of 95,000 dalton subunit of insulin receptor
S Tamura, TA Brown, RE Dubler, J Larner - Biochemical and Biophysical …, 1983 - Elsevier
S Tamura, TA Brown, RE Dubler, J Larner
Biochemical and Biophysical Research Communications, 1983•ElsevierVanadate enhanced the state of activation of rat adipocyte glycogen synthase in a manner
similar to that of insulin. No additional effect was observed when insulin and vanadate were
added together. The effect of vanadate, like insulin, was reversed by incubation with
epinephrine. Vanadate also enhanced the degree of phosphorylation of the 95,000 dalton
subunit of insulin receptor, selectively on tyrosine residues, in the solubilized rat adipocyte
insulin receptor system. This demonstrates that insulin and vanadate have similar initial …
similar to that of insulin. No additional effect was observed when insulin and vanadate were
added together. The effect of vanadate, like insulin, was reversed by incubation with
epinephrine. Vanadate also enhanced the degree of phosphorylation of the 95,000 dalton
subunit of insulin receptor, selectively on tyrosine residues, in the solubilized rat adipocyte
insulin receptor system. This demonstrates that insulin and vanadate have similar initial …
Abstract
Vanadate enhanced the state of activation of rat adipocyte glycogen synthase in a manner similar to that of insulin. No additional effect was observed when insulin and vanadate were added together. The effect of vanadate, like insulin, was reversed by incubation with epinephrine. Vanadate also enhanced the degree of phosphorylation of the 95,000 dalton subunit of insulin receptor, selectively on tyrosine residues, in the solubilized rat adipocyte insulin receptor system. This demonstrates that insulin and vanadate have similar initial actions on receptor phosphorylation and also act similarly on an intracellular event, namely the activation of glycogen synthase.
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