A specific enzyme-linked immunosorbent assay for measuring β-amyloid protein oligomers in human plasma and brain tissue of patients with Alzheimer disease
Objective To examine in vivo levels of β-amyloid (Aβ) oligomers (oAβ) vs monomeric Aβ in
plasma and brain tissue of patients with sporadic and familial Alzheimer disease (AD) using
a new enzyme-linked immunosorbent assay (ELISA) specific for oAβ. Design To establish
the oAβ ELISA, the same N-terminal Aβ antibody was used for antigen capture and
detection. Plasma and postmortem brain tissue from patients with AD and control subjects
were systematically analyzed by conventional monomeric Aβ and new oAβ ELISAs …
plasma and brain tissue of patients with sporadic and familial Alzheimer disease (AD) using
a new enzyme-linked immunosorbent assay (ELISA) specific for oAβ. Design To establish
the oAβ ELISA, the same N-terminal Aβ antibody was used for antigen capture and
detection. Plasma and postmortem brain tissue from patients with AD and control subjects
were systematically analyzed by conventional monomeric Aβ and new oAβ ELISAs …
