Somatic mutation of the T15 heavy chain gives rise to an antibody with autoantibody specificity.
B Diamond, MD Scharff - Proceedings of the National …, 1984 - National Acad Sciences
B Diamond, MD Scharff
Proceedings of the National Academy of Sciences, 1984•National Acad SciencesThe S107 IgA kappa-chain myeloma cell line makes an antiphosphocholine antibody of the
T15 idiotype. A somatic mutant of this line, U4, makes an immunoglobulin with a single
amino acid substitution of an alanine for a glutamic acid at residue 35. This single amino
acid change results in a loss of phosphocholine binding activity. However, the U4
immunoglobulin has acquired reactivity with a variety of phosphorylated macromolecules,
including double-stranded DNA, protamine, and cardiolipin. Thus, a single amino acid …
T15 idiotype. A somatic mutant of this line, U4, makes an immunoglobulin with a single
amino acid substitution of an alanine for a glutamic acid at residue 35. This single amino
acid change results in a loss of phosphocholine binding activity. However, the U4
immunoglobulin has acquired reactivity with a variety of phosphorylated macromolecules,
including double-stranded DNA, protamine, and cardiolipin. Thus, a single amino acid …
The S107 IgA kappa-chain myeloma cell line makes an antiphosphocholine antibody of the T15 idiotype. A somatic mutant of this line, U4, makes an immunoglobulin with a single amino acid substitution of an alanine for a glutamic acid at residue 35. This single amino acid change results in a loss of phosphocholine binding activity. However, the U4 immunoglobulin has acquired reactivity with a variety of phosphorylated macromolecules, including double-stranded DNA, protamine, and cardiolipin. Thus, a single amino acid change in the T15 heavy chain can transform an antibacterial antibody into an antibody that resembles the autoantibodies seen in mice and man with autoimmune disease.
National Acad Sciences