Isolation of human hepatocytes by a two-step collagenase perfusion procedure

SML Lee, C Schelcher, M Demmel, M Hauner… - JoVE (Journal of …, 2013 - jove.com
The liver, an organ with an exceptional regeneration capacity, carries out a wide range of
functions, such as detoxification, metabolism and homeostasis. As such, hepatocytes are an
important model for a large variety of research questions. In particular, the use of human
hepatocytes is especially important in the fields of pharmacokinetics, toxicology, liver
regeneration and translational research. Thus, this method presents a modified version of a
two-step collagenase perfusion procedure to isolate hepatocytes as described by Seglen 1 …