[HTML][HTML] Deep, quantitative coverage of the lysine acetylome using novel anti-acetyl-lysine antibodies and an optimized proteomic workflow*[S]
T Svinkina, H Gu, JC Silva, P Mertins, J Qiao… - Molecular & Cellular …, 2015 - ASBMB
Introduction of antibodies specific for acetylated lysine has significantly improved the
detection of endogenous acetylation sites by mass spectrometry. Here, we describe a new,
commercially available mixture of anti-lysine acetylation (Kac) antibodies and show its utility
for in-depth profiling of the acetylome. Specifically, seven complementary monoclones with
high specificity for Kac were combined into a final anti-Kac reagent which results in at least a
twofold increase in identification of Kac peptides over a commonly used Kac antibody. We …
detection of endogenous acetylation sites by mass spectrometry. Here, we describe a new,
commercially available mixture of anti-lysine acetylation (Kac) antibodies and show its utility
for in-depth profiling of the acetylome. Specifically, seven complementary monoclones with
high specificity for Kac were combined into a final anti-Kac reagent which results in at least a
twofold increase in identification of Kac peptides over a commonly used Kac antibody. We …