[HTML][HTML] TRUST4: immune repertoire reconstruction from bulk and single-cell RNA-seq data
We introduce the TRUST4 open-source algorithm for reconstruction of immune receptor
repertoires in αβ/γδ T cells and B cells from RNA-sequencing (RNA-seq) data. Compared
with competing methods, TRUST4 supports both FASTQ and BAM format and is faster and
more sensitive in assembling longer—even full-length—receptor repertoires. TRUST4 can
also call repertoire sequences from single-cell RNA-seq (scRNA-seq) data without V (D) J
enrichment, and is compatible with both SMART-seq and 5′ 10x Genomics platforms.
repertoires in αβ/γδ T cells and B cells from RNA-sequencing (RNA-seq) data. Compared
with competing methods, TRUST4 supports both FASTQ and BAM format and is faster and
more sensitive in assembling longer—even full-length—receptor repertoires. TRUST4 can
also call repertoire sequences from single-cell RNA-seq (scRNA-seq) data without V (D) J
enrichment, and is compatible with both SMART-seq and 5′ 10x Genomics platforms.
Abstract
We introduce the TRUST4 open-source algorithm for reconstruction of immune receptor repertoires in αβ/γδ T cells and B cells from RNA-sequencing (RNA-seq) data. Compared with competing methods, TRUST4 supports both FASTQ and BAM format and is faster and more sensitive in assembling longer—even full-length—receptor repertoires. TRUST4 can also call repertoire sequences from single-cell RNA-seq (scRNA-seq) data without V(D)J enrichment, and is compatible with both SMART-seq and 5′ 10x Genomics platforms.
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